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Fig. 7. Ultrastructural analysis of Shroom2 RPE. (A,B) High
magnification TEM allowed further analysis of Shroom2-SPL-MO morphant
melanosome morphology. (A) Control RPE contained smooth-edged melanosomes of
varying size and black because of melanin accumulation. (B,b') Two
representative regions of Shroom2-SPL-MO morphant RPE. Melanosome number was
similar to controls; however, melanosome morphology was severely abnormal.
Melanosomes contained numerous vesicular structures, most of which appeared to
have not coalesced into a single structure resulting in a `bunch of grapes'
morphology. (C) Measurement of relative melanosome area of melanosomes
in Shroom2-SPL-MO morphants and controls, indicated that the melanosome
aggregates of Shroom2 morphants were almost 90% larger in area than controls.
(D) Shroom2-SPL-MO morphant melanosomes were also found to have highly
irregular surfaces, with a relative roundness value of over 1.7 and compared
with a value of 1.36 for control melanosomes (a perfect circle has a roundness
of 1.00).
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