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Fig. 2. Analysis of TF gene expression in the developing GI tract according to
protein families. (A) TF gene families show divergent patterns of
expression and temporal modulation during intestine development, with
proportionally higher representation of basic-leucine zipper (bZip) and
zinc-binding (ZnB) factors. Nuclear receptor (NR), homeodomain (HD) and
high-mobility group (HMG) factors have the highest degree of temporal
modulation. Except for HD and NR, most families are distributed similarly in
intestine (In, shown here) and stomach (St, data are shown only for NR and
HD). (B) Comparative gene expression for a single TF subfamily,
Hox-cluster genes, commonly proposed as candidates for anteroposterior gut
patterning. Expression in the developing gut is limited to genes at the
3' ends of collinear clusters and, with the few exceptions marked in red
type, is very similar in extent and modulation in fetal stomach and small
intestine. (C) As a group, NRs showed the greatest increase during
intestine development, mostly from E11 to E13. One factor expressed with these
dynamics is Nr2e3, previously regarded as a photoreceptor-specific product.
(D) Nr2e3 mRNA, expressed in the developing gut, is abrogated
in adult mice. (E) Fetal expression of Nr2e3 is restricted to the
proximal small bowel. (F) In situ hybridization (E15 intestine) reveals
Nr2e3 expression in the epithelial compartment (arrows).
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