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Figure 7


Fig. 7. Altered intestinal gene expression in mice lacking Isx function. (A) Microarray data for increased Scarb1 mRNA levels in Isx-/- (KO) ileum compared with control (WT) littermates. Dark signals represent absence and yellow the presence of hybridization; in each set, the top row shows probes that perfectly match the target transcript and the bottom row shows probes with single-base mismatches. Similar results were obtained for two independent Scarb1-specific probe sets. (B) qPCR confirmation of significant elevations in Scarb1 mRNA in Isx-/- ileum and duodenum (Duod) but not in the other sites of Scarb1 expression, adrenal gland and liver, where Isx is absent. All mutant (KO) values are expressed in relation to the control (WT, assigned a value of 1.0) for that tissue. Scarb1 mRNA levels in liver and adrenal glands are higher than in intestine, but increases in Isx-/- mice are confined to the gut. (C) Immunoblot confirmation of elevated Scarb1 protein levels in Isx-/- intestine but not liver (data not shown) or adrenal glands. (D,E) Immunohistochemistry with Scarb1 antiserum. Wild-type ileum (D) reveals no specific signal as Scarb1 is primarily a duodenal product, whereas Scarb1 localizes (arrowheads) in the mutant (KO) ileal apical brush border (E).





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