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Figure 4


Fig. 4. wingless represses drm transcription in anterior eye disc margin. (A,B) Early and (C-F) late L3 discs. (A) In wg-Z discs (ß-galactosidase, orange), prior to the initiation of retinal differentiation, drm and wg expressions are complementary. (C) In late L3 discs, this complementarity is maintained with the exception of the appearance of a dorsal head drm-expressing patch (asterisk in C,D,F). (B) In early L3 wgCX3 discs, drm transcription extends dorsally to reach the antenna (black arrowhead) before retinogenesis starts. (D) In older discs, ectopic retinogenesis (red arrowhead) can be seen progressing from the drm-expressing anterior margin (black arrowhead). (E) Dorsal margin of an odd-GAL4/wg-Z; UAS-GFP L3 disc. odd reporter expression (green and outlined in by the green line in the single channel panels) is complementary to both wg transcription (wg-Z) and protein expression (Wg). (F) A late wild-type L3 disc stained for drm and Elav is shown for comparison. Discs are oriented with posterior towards the right and dorsal upwards.





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