QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Abnormal axon trajectories in the AC of COUP-TFI^null brains. Serial free-floating coronal (A-D') and horizontal (G-I') sections through the telencephalon of E18.5 wild-type and COUP-TFI^null fetuses immunostained for L1. In wild-type embryos, the anterior tract of the AC is visible as distinct spots in anterior coronal sections (A,B) and as a horseshoe-shaped tract in horizontal sections (H). The posterior tract is positioned more dorsally than the anterior one and forms a laterally directed tract connecting the two temporal lobes (G). The two tracts show decussation jointly at the midline (C,D,G). In the COUP-TFI^null brains, the anterior and posterior tracts form ectopic fascicles that innervate the rostromedial cortex (arrows in H',I'). The main tract shows decussation at the midline but its overall diameter is larger (arrowheads in C',D',G',H',I'). The arrows in A' and B' indicate ectopic fascicles of the anterior tract in rostral sections. (E-F') Double dye tracing of E18.5 brains in which a crystal of DiI was placed in the hippocampus (E,E') and a crystal of DiA was placed in the anterior branch of the AC (F,F'). Note that in the null embryos hippocampal-septal projections enter the AC tract (arrowhead in E') and the AC fibres project dorsally towards the hippocampal commissure (arrowhead in F'). (J) Quantification of the thickness of wild-type (blue) and null (red) AC measured at the midline. In the null embryos, the AC is significantly larger in both the coronal and horizontal planes. Scale bars: 200 µm. acA, anterior tract of the AC; acP, posterior tract of the AC; NULL, COUP-TFI^null fetuses; WT, wild-type fetuses.

Return to article