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Fig. 5. Altered gene expression and protein levels of actin and microtubule cytoskeleton molecules in COUP-TFI^null. (A) Fold-change variations (expressed as 2^-Ct values) in MAP1B, MAP2, Rnd2 and CAP1 mRNA expression levels between the wild-type and COUP-TFI^null E14.5 brains after QRT-PCR. The data are normalized to levels of expression of all of the genes in wild-type embryos as 1.0. Significant differences are indicated by asterisks. (B) Western blots of E14.5 brain extracts from wild-type and COUP-TFI^null littermates. The blots were incubated with the antibodies listed on the right-hand side. (C-E') Coronal sections of E15.5 wild-type and COUP-TFI^null dorsolateral cortices immunostained for MAP1B, RND2 and TAU antibodies. (C',D') The arrows indicate reduced MAP1B and increased RND2 protein levels in the upper intermediate zone, respectively. The asterisks show altered expression in the marginal zone and the arrowhead in D' points to an expanded expression of RND2 into more dorsal regions. (E,E') The arrowheads show no changes in expression levels of the general microtubule-associated protein TAU. Scale bar: 200 µm. IZ, intermediate zone; MZ, marginal zone; NULL, COUP-TFI^null fetuses; VZ, ventricular zone; WT, wild-type fetuses.

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