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Fig. 7. Oral expression of foxa is required for mouth formation.
Red indicates foxa MASO-injected cells; green indicates control
cells. (A,D) Diagram of the experiment. Reciprocal combinations
of animal and vegetal halves were recombined. (B,C) Control
animal half, showing normal mouth (arrowhead, C). The foxA MASO
vegetal half (red) produces a truncated gut, with most of the red cells
becoming SMCs, and extra pigment cells (compare black cells in C and F).
(E,F) foxa MASO-injected animal half (red) produces
ectoderm with no mouth. The control vegetal half (green) produces a normal
gut, including foregut that leads to a dead end. (B,E) DIC images superimposed
on fluorescent images. (C,F) Fluorescent image only at a slightly higher
magnification, focused on the mouth region.