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Fig. 3. Effects of overexpressing MIR167 genes. (A)
Sequences of miR167 target sites on ARF6 and ARF8
mRNA, and the mutated target sites of mARF6 and mARF8. The
Watson-Crick base pairings to the miR167 sequence are shown.
G
(kcal/mol) was calculated by Mfold (Zuker,
2003). Mutated nucleotides are in lower case. (B)
Phenotypes of plants overexpressing four different MIR167 genes. From
left to right: wild type (WT), arf6/+ arf8, arf6 arf8,
P35S::MIR167a (a), P35S::MIR167b
(b), P35S::MIR167c (c) and
P35S::MIR167d (d). (C) Stage 13 flowers of
P35S::MIR167 plants. Genotypes are the same as in B.
(D) Northern blot analyses of MIR167-overexpressing transgenic
plant flowers. U6 snRNA and ß-tubulin are included as loading controls.
Numbers beneath lanes indicate relative transcript levels normalized to
loading controls.