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Figure 1


Fig. 1. C/EBP{alpha} and its mRNA expression in mouse liver development. Immunohistochemistry reveals that hepatoblasts and hepatocytes express C/EBP{alpha}, localized in their nuclei, in 12.5-(A,B), 14.5-day (D) and neonatal (F) liver sections. (B) Double staining of C/EBP{alpha} and DAPI. (C,E) Cytokeratin immunostaining of D and F, respectively. Pearl-like structures and biliary epithelial cells are negative for C/EBP{alpha} (arrows in C-F). In situ hybridization using Cebpa antisense probes shows the absence of Cebpa mRNA in biliary epithelial cells (arrows) of 16.5-day (G) and 17.5-day (I) mouse liver sections. Hepatocytes are positive for Cebpa mRNA. Sense probes give no significant signal on a 16.5-day liver section (H). PV, portal vein. Scale bars: 50 µm.





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