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Fig. 8. In situ hybridization analysis of Jag1 and Notch2
mRNAs in fetal and neonatal livers. In the wild-type liver, hepatoblasts
very weakly express Jag1 and Notch2 mRNAs at 12.5 days
(arrows; A,B,G,H), but epithelial cells of
periportal biliary structures become positive for both mRNAs in later
development (arrows; C-F,I,K,L). Epithelial cells
of the extrahepatic bile duct are moderately positive for both mRNAs
(A,G,J). Endothelial cells of the portal vein and hepatic artery
(*; E), and their connective tissue cells express Jag1
mRNA (A-F). Arrowheads indicate Jag1 mRNA-positive hemopoietic cells
in the neonatal wild-type liver (F). In the 12.5-day knockout liver, although
Jag1 mRNA expression in hepatoblasts is at a normal level (arrow;
M), Notch2 mRNA is upregulated in hepatoblasts (arrow;
Q). Hepatoblasts under the capsule (large arrowheads) are positive for
Notch2 mRNA (R). In 15.5-day and neonatal knockout livers,
Jag1 and Notch2 mRNAs are upregulated in pseudoglandular
structures (star), but periportal epithelial cells are more strongly stained
(small arrowheads; N-P,S,T). Jag1 mRNA
expression is downregulated in endothelial cells of the portal vein (N),
compared with that in the 17.5-day wild-type liver (C,D,E). ED, extrahepatic
bile duct; PV, portal vein. Scale bars: 50 µm.
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