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Figure 5


Fig. 5. Change in size of hepatocyte colonies. Morphometric analysis using Image-Pro Plus software showed approximately two- to fourfold increase in the cross-sectional area of DPPIV+ hepatocyte colonies over a 6-month period. P<0.0001 when the area of colonies derived from CMP-FLECIII (isolate R) were compared using Kruskal Wallis ANOVA, indicating that increase in size was statistically significant. P values generated using Bonferrioni adjusted Mann-Whitney test to compare areas at different time points showed that the increase in area of hepatocyte colonies derived from CMP-FLECIII (P<0.001 for 3 weeks versus 3 months; P=0.009 for 3 weeks versus 9 months; P=0.06 for 3 months versus 9 months) and CMP-FLECIV (isolate P) (P<0.0001 for 3 months versus 9 months) was statistically significant. Sample size at 9 month time point for CMP-FLECIII colonies was small because of the reduction in discrete colonies produced by merging, a reduction that increased both P values and standard error of the mean.





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