|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Aberrant centrosome behavior in pam-1. (A) Centrosome
positions over time, measured from time-lapse images of embryos expressing
ß-TUBULIN::GFP. Each point represents the average of six wild-type or
seven pam-1 embryos beginning with the moment of the appearance of
the centrosomes. In pam-1 embryos, centrosomes first became visible
in the posterior of the embryo, but then quickly moved toward the center of
the embryo. (B) A plot of centrosome position relative to the posterior
pole from three wild-type and three pam-1 embryos over ten 20 second
time points. Each horizontal series represents measurements from one embryo.
Measurements were begun when the centrosomes first became visible. Stars
indicate the position of the centrosomes when they first separated from one
another. The centrosomes in pam-1 moved quickly toward the center of
the embryo. (C, parts a-c) Images from a time-lapse ß-TUBULIN::GFP
movie of a wild-type embryo show movement of the centrosomes over time. By 200
seconds, the centrosomes clearly flanked the sperm pronucleus near the
posterior pole of the embryo. (C, parts d-f) In pam-1 the centrosomes
moved quickly into the center of the embryo and remained close together before
appearance of the pronuclei. (D, parts a-c) By DIC optics, the
centrosomes appeared as small dots flanking the pronuclei until the first
spindle assembled (white arrowheads). (D, part d) By contrast, in some
pam-1 embryos, the centrosomes began to nucleate microtubules before
pronuclear appearance and were visible by DIC optics (white arrowheads). (D,
parts e-f) In most cases, the centrosomes reduced in size as the pronuclei
formed and then grew larger again as the mitotic spindle assembled. Scale bar:
10 µm.
|
