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Figure 3


Fig. 3. Defects in brain NB pattern in vnd mutants at stage 11. (A-F) Antibody double staining against Engrailed [En; En-lacZ (A,B), En-protein (C-F)] and Deadpan (Dpn; A-D) or Seven up-lacZ (Svp-lacZ; E,F) in wild type (A,B,E) or in vnd mutants (C,D,F). (A,B) All brain NBs have developed by stage 11. (B) Higher magnification of region framed in A. NBs delaminating from the en head spot (hs), the en antennal stripe (as) and the en intercalary stripe (is) are indicated. (C) Broken yellow line encloses ventral pNE, in which NB formation is abnormal. (D) Higher magnification of the region framed in C; Dv8, Tv4,5 are missing. (E,F) All ventral NBs (including the ventral Svp-lacZ-positive) are indicated in white or green, dorsal Svp-lacZ-positive NBs in black inscriptions. (F) In vnd mutants, ventral NBs indicated in green in E are missing. Segmental borders between the trito-(TC), deuto-(DC) and protocerebrum (PC) are marked by broken red lines. as, en antennal stripe; hs, en head spot; is, en intercalary stripe; AN, MD, MX, antennal, mandibular, maxillary segment, respectively; CL, clypeolabrum; FG, foregut. Anterior is upwards.





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