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Figure 1


Fig. 1. Spatiotemporal expression of vnd during embryonic brain development. Laser confocal microscopy, reconstructions of optical sections, lateral views (A,C) and 3D reconstructed models of confocal microscopic stacks, covering corresponding optical sections (B,D) are shown for embryonic late stage 12 (A,B), and stage 15 (C,D). (A) Embryo double-immunolabelled with anti-Neurotactin (NRT) antibody (red) and anti-VND antibody (green/yellow). (C) Double-immunolabelling with anti-HRP antibody (red) and anti-VND antibody (green, yellow). (A,C) At stage late 12, three vnd expression domains become apparent that are still observable at stage 15. (B,D) 3D reconstructed models show relative location of domains within developing brain (vnd expression domains: blue, protocerebral; green, deuterocerebral; red, tritocerebral).





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