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Fig. 3. Defective neuroblast formation in lab-expressing tritocerebral
domain of vnd mutants. (A-D) Double-immunolabelling with
neuroblast-specific anti-DPN (blue) and anti-LAB (brown), at embryonic stage
11, in wild type (WT) (A,B) and vnd-null mutants (C,D). (A-D) Ventral
views of flat preparations. (B,D) Higher magnification of regions indicated in
A,C by black frames, at level of brain neuroblasts. (A) In wild-type embryos,
all brain neuroblasts have developed by stage 11. (B) Two deutocerebral and
complete set of tritocerebral neuroblasts developing from LAB domain are
indicated [according to nomenclature of Urbach et al.
(Urbach et al., 2003)]: Tv1-5,
ventral tritocerebral neuroblasts; Td1-8, dorsal tritocerebral neuroblasts;
Dv2, Dv4 ventral deutocerebral neuroblasts. Broken line encircles group of
dorsal neuroblasts that are assumed to be retained in vnd-null
mutants (compare with D). (C) In vnd-null mutants, overall expansion
of LAB domain appears to be reduced when compared with wild type (A), and
invagination of the foregut (Fg) is affected (compare lateral extension of
foregut invagination as marked by red arrowheads in A and C). (D) Number of
DPN-positive neuroblasts (white asterisks) is diminished, when compared with
B. One neuroblast (white dot) does not express DPN at detectable levels;
similarly, in wild type a neuroblast expressing DPN at significantly lower
levels is found in same relative position (see Td5 in cluster of neuroblasts
encircled by broken line, B). Fg, foregut; Lr, labrum; Md and Mx, mandibular
and maxillary segment, respectively.
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