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Figure 6


Fig. 6. Partial restoration of brain structures and lab expression in vnd mutants by blocking apoptosis. Laser confocal microscopy of stage 15 embryos, reconstructions of optical sections, lateral views. Arrows indicate tritocerebral region. (A) Wild type; (B) vnd mutant; (C,D) sca::Gal4/UAS::p35 in vnd null mutant background. (A-C) Embryonic brain immunolabelled with anti-HRP (red). (D) Embryonic brain double-immunolabelled with anti-HRP (red) and anti-LAB (green). When compared with wild-type (A) and vnd mutant brain (B), p35-mediated block of apoptosis partially restores HRP-immunoreactive tissue in vnd mutant tritocerebrum, and descending longitudinal connectives that transverse the tritocerebrum are detectable (C). Restoration of neural tissue also results in wild-type-like lab expression domain in vnd mutant tritocerebrum (D, compare with Fig. 4B).





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