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Fig. 7. Partial restoration of brain tracts in cell death prevented vnd
mutant. Laser confocal microscopy of stage 15 embryos, reconstructions of
optical sections, lateral views. Arrows indicate tritocerebral region.
(A,B) Wild-type; (C,D) vnd mutant;
(E,F) sca::Gal4/UAS::p35 in vnd-null
mutant background. Embryos in B,D,F are not the same as in A,C,E,
respectively. (A,C,E) Embryonic brain immunolabelled with anti-FAS2 (red).
(B,D,F) Embryonic brain double-immunolabelled with anti-FAS2 (red) and
anti-ELAV (green). (A,B) In wild type, FAS2 immunostaining reveals a number of
early differentiating neurons as well as axon tracts, and ELAV expression is
apparent within postmitotic neurons of all brain neuromeres, including the
tritocerebrum. (C,D) By contrast, in vnd-null mutants, a gap is seen
in the area of the tritocerebral region and the majority of ELAV-expressing
cells are lacking in this domain. (E,F) Ubiquitous block of apoptosis
partially restores the gap-like defects observed in vnd
loss-of-function mutants: FAS2-immunoreactive longitudinal connectives are
detectable, although neural fibres display fasciculation defects, and a
significant number of ELAV-expressing cells are detectable in the cell death
prevented vnd mutant tritocerebrum (F, arrow; compare with B).
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