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Figure 2


Fig. 2. IP3R1 is differentially phosphorylated in Xenopus eggs and zygotes. (A) Western blotting performed on Xenopus egg extracts (approximately three eggs/lane) collected at MII and at interphase (Int) shows MPM2 (upper panel) and IP3R1 reactivity (lower panel). (B) Immunoprecipitation (IP) experiments performed on MII Xenopus egg extracts using preimmune serum (Preim.), anti-IP3R1 antibody, MPM2 antibody or beads alone, followed by SDS-PAGE and western blotting with the anti-IP3R1 antibody (upper panel) and the MPM2 antibody (lower panel).





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