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Fig. 10. TrkB expression in OB, and Bdnf action on OBSC-derived neurons.
(A-E) Coronal sections from P6 and P21 mice were stained with an
antibody to TrkB (A, ML), (B, GL), or double-stained with anti-TrkB and
anti-SV2 (C-E), showing that in addition to expression in neuronal cell
bodies, TrkB is highly expressed in synaptic terminals at the glomeruli.
(F-Q) E13.5-derived OBSC cultures were grown for 15-17 days.
Representative fields of TuJ1+ neurons (F,G) and synapsin
I+ neurons (H,I) in control (F,H) and Bdnf-treated (G,I) cultures.
Representative fields of GABA+/SV2+ (J-M) and
Th+/SV2+ neurons (N-Q) in control (J,K,N,O) and
Bdnf-treated (L,M,P,Q) cultures. Note that Bdnf-treated neurons show more
synapsin I+ and SV2+ boutons than controls. Similar
results were obtained in three experiments. Scale bar in Q: 35 µm for A,B;
25 µm for C-E; 30 µm for F-I; 15 µm for J-Q.
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