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Fig. 2. Gsh2 and Dlx2 expression and interneuron generation in short-term
dissociated cultures of E13.5 OB and E13.5 GE. Cell suspensions of E13.5
OB and GE were cultured for 6 days. Representative fields of Gsh2/GAD-positive
cells (OB, A-C; GE, G-I) and Dlx2/GAD-positive cells (OB,
D-F; GE, J-L) in cultures. Arrows indicate double-labeled cells;
arrowheads show GAD+ neurons that do not express Gsh2 or Dlx2.
(M) Percentages of GAD+ neurons over total TuJ1+
neurons. (N) Percentages of Gsh2- or Dlx2-expressing GAD+
cells relative to total GAD+ cells. (O) Percentages of Gsh2-
or Dlx2-expressing GAD+ cells relative to total Gsh2+ or
total Dlx2+ cells. No (0%) Gsh2+ cell coexpressed GAD in
OB cultures; Student's t test could thus not be calculated.
*P<0.05 (OB versus GE). Results are the
mean±s.e.m. of data from six cultures in three experiments. Scale bar
in L: 25 µm.
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