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Fig. 6. Transplant of E13.5 OB and E13.5 LGE precursor cells expressing GFP in
P5-P7 wild-type OB. E13.5 OB (A-E,K,L) and LGE
(F-K,L) cell suspensions were transplanted into the OB SEZ of P5
and P7 mouse pups. Grafted animals were analyzed 1 to 4 weeks after surgery.
(A) One week post-transplant, GFP-expressing OB cells with a migratory
morphology were observed in host tissue. (B,C) By 2 and 4 weeks, transplanted
GFP-positive cells migrated away from the injection site to the GCL, the IPL,
the boundary between the IPL and the ML, and the GL. (C,K) Most grafted OB
cells reached the GCL and the limits established by the IPL and the EPL (ML +
PL). (F-K) LGE-derived GFP+ cells migrated and differentiated
within the OB; they migrated preferentially to the GCL and GL, with fewer
cells reaching the ML + PL (G,H,K). The numbers of cells located in the
different cell layers were counted and expressed relative to total
transplanted GFP+ OB or GFP+ LGE cells found in the
postnatal OB 2-4 weeks post-transplant (K). Results are the
average±s.e.m. of data from mice receiving OB cells (n=4 mice)
or LGE cells (n=4 mice). *P<0.05 (OB vs LGE;
Student's t test). (D-J,L). Morphological analysis was performed on
50 neurons derived from GFP+ OB precursor cells (n=3
mice), and on 50 neurons from GFP+ LGE precursors (n=3
mice). Percentages of neurons with one apical dendrite, two apical dendrites
or more than two dendrites are given. Note that the great majority of neurons
found in mice transplanted with OB cells had one or two apical dendrites
(D,E,L). Neurons with one apical dendrite were also abundant in mice
transplanted with LGE cells (I,L). In these mice, however, the percentage of
neurons possessing two apical dendrites, and of neurons that were
multidendritic (J) was 2.3 times fewer and 4.1 times greater, respectively,
than in mice grafted with OB cells *P<0.05 (OB vs LGE;
Student's t test). m, more than two dendrites; o.a.d., one apical
dentrite; t.a.d., two apical dendrites; GCL, granule cell layer; IPL, internal
plexiform layer; ML, mitral cell layer; EPL, external plexiform layer; GL,
glomerular layer. Scale bar in J: 90 µm for A-C,F,H; 50 µm for D-I; 20
µm for G; 15 µm for J.
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