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Fig. 7. Differentiation and maturation of E13.5 OB precursor cells expressing
GFP in P5-P7 wild-type OB. E13.5 OB cell suspensions were transplanted and
mice were analyzed 4 to 10 weeks after surgery. (A) Many granule neurons had a
single, large apical dendrite that crossed the ML and extended dendritic
branches with spines in the EPL. The granule neurons at the IPL-ML boundary
(arrow in A) had two major apical dendrites that also extended branches with
spines into the EPL (A,C,D, D inset). Staining with an
anti-synaptophysin antibody (E,F) revealed close proximity of
dendritic spines to synaptic boutons. (B) As host periglomerular
neurons (inset in B shows a periglomerular/glomerular area stained for Th),
some transplanted cells oriented their cell bodies horizontally. (G-O)
Sections were stained with anti-GAD or anti-GABA antibodies. In some neurons,
GAD or GABA and GFP colocalization was best observed in specific areas of the
cell body. (P-R) The Th+ cell body surrounds the nuclear
GFP+ signal (arrows). Neuron migration and differentiation was
observed in all transplanted mice (n=6). Scale bar in R: 20 µm for
A-C; 15 µm for D,G-I; 4 µm for D inset; 10 µm for E,F,J-R.
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