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Fig. 8. OBSC differentiation into GABAergic neurons. (A-G) E13.5- and
14.5-derived OBSC cultures were grown for 15-17 days in the absence of
mitogens. Bdnf (20 ng/ml) was added to some cultures. Cells were
double-immunostained with anti-GAD and anti-Dlx2 (A,B) and anti-MAP2ab and
anti-GABA antibodies (C-F). (A,B) GAD+/Dlx2+ cells
(arrows) and GAD+/Dlx2- cells (arrowheads) in the
differentiating cultures. (E,F). Some Bdnf-treated cells showed a more complex
neuronal morphology than controls. (G) Average percentages of GABA+
neurons relative to total MAP2ab+ neurons. Results are the
mean±s.e.m. of data from four cultures in two experiments (passages
3-5). (H) Single cells were cultured to produce clonal neurospheres
(I) which, after differentiation (J), generated GABA+
neurons (K,L). Scale bars in F and L: 40 µm for A-F; 9 µm
for H; 30 µm for I,J; 20 µm for K,L.
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