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Figure 2


Fig. 2. Plasmid electroporation of linear heart tubes. Stage 12 hearts were dissected, electroporated with RSV promoter cDNA constructs to direct production of epitope-tagged Notch pathway modulators or GFP (see Materials and methods). During the subsequent 24-hour culture period, the electroporated hearts looped and developed appropriate expression patterns of region-specific markers, including Cx43, vmhc and amhc in chamber myocardium, as well as Bmp2 in the AVC. In addition, Serrate1 and Hey2 showed characteristic expression patterns. Inset in Bmp2 panel shows Cy3 immunofluorescent detection of the Myc-epitope tag-labelled product of transfected eGFP gene. a, atrial region; avc, atrioventricular canal; ic, inner curvature; ot, outflow tract; v, ventricular region.





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