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Figure 7


Fig. 7. Tbx2 represses Hey1 and Hey2 cell autonomously. (A) Misexpression of Myc-tagged Tbx2 in stage 12 hearts caused a cell-autonomous reduction in the incidence of Hey1- and Hey2-positive cells. Tbx5, by contrast, had no effect. Asterisks indicate statistical significance of the difference between electroporated Tbx2 and the GFP control (P<0.05, {chi}2 test). (B,C) Histological sections demonstrating the cell-autonomous suppression of endogenous Hey1 (B) and Hey2 (C) by electroporated Tbx2. (D) Flag-tagged mouse Hey1 and mouse Hey2 did not alter Tbx2 expression. Brightfield, merged and fluorescent images are as in Fig. 3. Scale bar: 50 µm.





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