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Figure 1


Fig. 1. Activation and repression by DL variants in S2 cells. (A) Structure of DL variants. (B) Activation of the D4-luciferase reporter by addition of increasing amounts (50 ng, 150 ng) of vectors encoding DL variants. (Inset) Anti-DL immunoblot of lysates of S2 cells transiently expressing the FLAG-DL constructs. (C) Synergistic activation of the DE5-luciferase reporter by vectors encoding DL variants (60 ng) and TWI (20 ng). An expression vector encoding GRO (500 ng) was also included in the indicated transfection mixtures. (D) Synergistic activation of the G5DE5-luciferase reporter by vectors encoding DL variants (60 ng) and TWI (20 ng). gro dsRNA (1 µg) was also included in the indicated transfection mixtures. (Inset) Anti-GRO immunoblot of lysates of S2 cells treated or not treated with gro dsRNA.





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