DEV02618 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. The M/+ to M⁺ transformation in the esg-M⁺ experiment affects all imaginal discs. (A) CNS and imaginal discs of a M(3)67C ubi-GFP /+ larva stained for GFP and topro-3 (red). All the structures express GFP and topro-3. (B) CNS and imaginal discs of an esg-Gal4; M(3)67C ubi-GFP FRT2A/mwh jv FRT2A larva stained in the same way as in A. All the imaginal discs (and part of the larval brains) have lost the GFP label and are therefore M⁺.

• Supplemental Figure 2 -

  Fig. S2. Apoptosis does not play a role in controlling final size in esg-M⁺ discs. (A) A prepupal wing disc stained for wg (blue) and caspase 3 (red) showing the normal low levels of apoptosis. (B) Adult esg-M⁺ wing showing a normal pattern, except for the radius incompletus phenotype caused by the ri mutation in cis in the mwh jv chromosome. (C) Prepupal esg-M⁺ p35 wing disc showing high levels of P35 (red). (D) Adult wing of the same phenotype showing normal size. There is an exacerbation of the ri phenotype due to P35.

• Supplemental Figure 3 -

  Fig. S3. Apoptosis levels. Apoptosis levels were determined in M/+ (A), ci-M⁺ (B) and en-M⁺ (C) discs. Staining for TUNEL (red) or anti-caspase 3 (blue) reveals no significant increase in apoptotic levels in any of these genotypes. (D) Late third instar en-M⁺ p35 disc showing groups of cells with strong GFP expression. We believe they are M/M cells that are maintained alive by p35 throughout larval development, even though they do not divide. (E) Wing of the same genotype as D, showing a normal size and pattern except for the shortening of vein 5, a distinctive feature caused by P35.