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Fig. 2. Lineage tracing analysis in the retina. All retinal sections were examined at 3 weeks of age. (A) X-gal staining of retinal sections from Ptf1a^Cre/+;R26R pups counterstained with Fast Red. No ß-gal activity was detected in the ONL. (B-L) Ptf1a^Cre/+;R26R-EYFP retinal sections double-labeled with anti-GFP (green) and the indicated antibodies (red). Brn3b (ganglion cells, B); Chx10 (bipolar cells, C); recoverin (rods and cones, D); calbindin (horizontal and amacrine cells, E); syntaxin (horizontal and amacrine cells, F); Gad65 (GABAergic amacrine cells, G); GABA (GABAergic amacrine cells, H); calretinin (amacrine subclass and ganglion cells, I); tyrosine hydroxylase (Th, dopaminergic amacrine cells, J); glycine transporter 1 (Glyt1, glycinergic amacrine cells, K); Cralbp (RPE and Müllar glia, L). Arrows in A,C,E indicate representative mature horizontal cells; arrowheads in E,H indicate non-colocalized cells. Scale bars: in A, 25 µm for A; in L, 25 µm for B-L. Abbreviations: INL, inner nuclear layer; ONL, outer nuclear layer; OPL, outer plexiform layer; GCL, ganglion cell layer; IPL, inner plexiform layer.

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