QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Defects in laminar structure and cell specification in Ptf1a mutant retinas. (A-F) X-gal staining of retinal sections from E18.5 Ptf1a^Cre/+;R26R embryos (A) and Ptf1a^Cre/Cre;R26R embryos (B) counterstained with Fast Red. (C,E) Higher-magnification images of the areas in the solid and broken rectangles in A, respectively. (D,F) Higher-magnification images of the areas in the solid and broken rectangles in B, respectively. Arrows in E indicate representative developing horizontal cells and the bracket in E indicates normal location of maturing amacrine cells in the E18.5 retina. The bracket in F indicates lacZ-positive nerve fiber layer, which is thicker than the control lacZ-negative nerve fiber layer (indicated by the small bracket in E). (G-J) Hematoxylin and Eosinstained sections of wild-type and mutant retinas at E18.5. Higher-magnification pictures of G and H are shown in I and J, respectively. Arrowheads in H indicate a front-line of cells in the outer neuroblastic layer invading into the GCL. (K,L) Wild type and Ptf1a^Cre/Cre E18.5 retinal sections were immunostained with an anti-Brn3b antibody. Scale bars: in B, 100 µm for A,B; in H, 50 µm for E-H; in D, 25 µm for C,D; in L, 25 µm for K,L; in J, 10 µm for I,J. (M) Top, increased ganglion cell number in Ptf1a^Cre/Cre retinas. Bottom, no significant change in central retinal thickness in the mutant. Histograms represent the mean±s.d. of measurements from five animals of each genotype. ^*P<0.001. Abbreviations: GCL, ganglion cell layer; IPL, inner plexiform layer.

Return to article