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Fig. 1. Effect of scf RNAi on expression of SCF and viability. (A)
Comparison of the RNAi effect on expression of SCF between males and females.
To generate control individuals (carrying only GAL4) or RNAi-induced
individuals (carrying both GAL4 and UAS-IRscf), the
Act5C-GAL4 driver was crossed to yw or yw;
[UAS-IRscf] (line 2-1) at 25°C (lanes 1 to 8) or 18°C (lanes
9 to 12). Two individuals (late third-instar larvae) of each sample type
indicated were randomly selected. Proteins in a 12 µl or 1.5 µl aliquot
of the extract from each animal were resolved by SDS-PAGE, blotted onto a
membrane, and the membrane probed with anti-SCF (upper panel) or
anti-
-tubulin antibody (lower panel, loading control). (B)
Effect of scf RNAi on viability of males and females. Each
UAS-IRscf line indicated (balanced with CyO, y+
or TM3, y+) was crossed to Act5C-GAL4
driver. F1 animals carrying only GAL4 (control), or carrying both
GAL4 and UAS-IRscf (RNAi), were sorted and counted. Numbers
above the bars indicate viability relative to the control sisters carrying
only GAL4. The reduction in male viability observed at 25°C was
significantly suppressed at 18°C in the experiment using the 2-1 line. The
number of control sisters examined for the lines 2-1, 3-1, 3-2, and 2-1 at
18°C was 180, 164, 59 and 160, respectively.
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