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Fig. 3. Localization of SCF along the dosage-compensated male X chromosome.
(A-E) Extra SCF signals are associated with the male X chromosome when
compared with autosomes or the female X chromosomes. Polytene chromosomes
prepared from males (A-C) or females (D,E) were stained with antibodies or
DAPI as indicated. Arrowheads indicate examples of the intensely stained SCF
bands, which are identical in males and females. Note that the `milky
way'-like signals between the intense bands are specific to the male X
chromosome. (F-I) Most of the faint signals (but not the intense bands)
of SCF colocalize with the MSL complex. Boxed regions in B and C are enlarged
in F and G, and merged and split images are shown in H and I, respectively.
(J-M) MSL-1 localization (J,K) and acetylation of histone H4K16 (L,M)
along the male X chromosome are not affected by knockdown of SCF (J,L). To
generate control individuals (carrying only GAL4) or RNAi-induced
individuals (carrying both GAL4 and UAS-IRscf), the
Act5C-GAL4 driver was crossed to yw or yw;
[UAS-IRscf] (line 2-1) at 25°C. Polytene chromosomes from RNAi
(J,L) or control (K,M) third-instar larvae were stained with DAPI (green in
J,K) and anti-MSL-1 antibody (magenta in J,K), or with anti-acetylated H4K16
antibody (L,M). (N,O) The presence of SCF on the male X
chromosome is reduced in a mof1 mutant background.
Polytene chromosomes were labeled with DAPI (green in N) and anti-SCF antibody
(O; magenta in N). The tip of the X chromosome is marked by an arrow, and the
X chromosome is boxed.
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