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Fig. 2. Insertion of newly recycled AChRs matches the removal of recycled
receptors. (A) The sternomastoid muscle was labeled with BTX-biotin
followed by a saturating dose of strept-488. Three to 4 days later, the animal
was anaesthetized and the sternomastoid muscle bathed with a saturating dose
of strept-594 to specifically label the recycled receptors; superficial
synapses were imaged immediately, and again 2 days later, and the loss of
recycled receptors determined. The sternomastoid muscle was then bathed with
fresh strept-594, and the synapses re-imaged to determine the insertion of
newly recycled AChRs. Scale bar, 20 µm. (B) Bar graph summarizing
the recycled AChR loss and insertion results (±s.d.) obtained by the
approach shown in A. Note that nearly all of the AChRs from the recycled pool
lost over the 2 days were replaced by newly recycled receptors re-inserted
into the postsynaptic membrane of the NMJ.
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