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Fig. 6. Impaired JNK signal in RET S697A mutant mice and impaired
chemoattractant response of ENCCs by the JNK inhibitor. (A) DRG was
dissected wild-type and homozygous RET S697A mutant mice at postnatal day 5
and cultured for 72 hours. Lysates were prepared from DRG neurons untreated or
treated with GDNF (20 minutes) and were analyzed by western blotting with the
indicated antibodies. In the panel of anti-pJNK, an asterisk indicates a
non-specific band. Two phosphorylated JNK bands were detected (arrows).
(B) Gut segments were dissected out from wild-type mice at E11.5, and
cultured with GDNF-soaked beads in the absence or presence of the JNK
inhibitor SP600125. After 3 days of culture, immunostaining with anti-PGP9.5
was carried out. Scale bars: 500 µm. (C) PGP9.5-positive area was
measured in the towards and away quadrants. Six explants for each experiment.
*P<0.01 compared with control.
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