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Fig. 7. Inhibition of migration of ENCCs in gut culture by kinase
inhibitors. Embryonic guts were dissected out from wildtype mice at E11.5
and cultured in control medium (A), in medium with MEK1 inhibitor
PD98059 (B), PI3K inhibitor LY294002 (C), JNK inhibitor SP600125
(D), PKA inhibitor KT5720 (E) and SRC kinase inhibitor SU6656
(F). After 3 days of culture, ENCCs were visualized by immunostaining
with anti-PGP9.5 antibody. Each experiment was carried out at least twice
using more than six embryonic guts. Arrows, the front of the migrating ENCCs;
arrowheads, cecum; asterisks, terminus of the hindgut; bars, 200 µm. Insets
show highpower views of the front of migrating ENCCs in dotted boxes of each
panel. To quantify inhibitory effects, the ratios of the distance of ENCC
migration to the colon length were measured and calculated. The ratios are
shown in the upper right corner of each panel.
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