DEV02642 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Playback of the image stack shown in Fig. 8B and in Movie 4 (in the supplementary material) revealed shear across the field, with actin bundles each advancing left to right above the other. We reregistered the image stack of Fig. 8B to an arbitrary reference object in one corner of the frame. By then plotting the lateral motion of individual fibers as a function of axial distance from this reference object (i.e. along an arbitrary animal-vegetal meridian; e.g. the white line in Fig. 8B), we found that each microfilament bundle advances incrementally past adjacent bundles in the same direction; no fibers undergo retrograde motion.

• Movie 1 -

  Movie 1. Time-lapse movie of chiral first cleavage induced by exposure to 20 mM BDM. Frames recorded 12 seconds apart.

• Movie 2 -

  Movie 2. Time-lapse showing cortical torsion of parthenogenetically activated egg treated with 20 mM BDM. The two pauses in the rate of torsion correspond to surface contraction waves associated with cell cycles (about 30 minutes apart). Frames recorded 12 seconds apart.

• Movie 3 -

  Movie 3. Side view of BDM-treated egg undergoing torsion. This is the egg used in the analysis described in Fig. 6. An image-sharpening filter was used to accentuate graininess to facilitate tracking of individual surface particles from frame to frame. Frames recorded 12 seconds apart.

• Movie 4 -

  Movie 4. Confocal time-lapse segment (corresponding to Fig. 8B and Fig. S1) showing GFP-actin-labeled microfilaments in small region of equatorial cortex. The size of the entire frame is 73 μm×73 μm. Frames 6 seconds apart.