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Fig. 11. Cortical f-actin in the dorsal marginal zone reorganizes to form a
contractile equatorial band following BDM treatment. (A) Embryos
were exposed to 20 mmol/l BDM beginning at 55 minutes post-fertilization and
fixed 5, 10 and 20 minutes later (60, 65, 70 minutes post-fertilization;
A1-A3, respectively). Each develops a horizontal sulcus, indicating
surface contraction, on the side opposite the SEP (prospective dorsal marginal
zone) (arrows in A2, A3). (B) Phalloidin-stained zygote, fixed at 75
minutes (20 minutes after BDM exposure) viewed in whole-mount via confocal
microscopy; same orientation as zygotes in A. Rhodamine-phalloidin (red)
stains equatorial sulcus brightly. Green reflection mode provides background,
primarily to indicate that cortical pigment is not obscuring the view of
cortical actin. (C) Detail of the surface at the edge of the sulcus,
showing equatorially aligned microfilaments. Scale bar: 60 µm.
(D,E) Animal and vegetal surfaces, respectively, of untreated
zygote, fixed 75 minutes post-fertilization and stained with
rhodamine-phalloidin, showing enrichment of cortical f-actin on the dorsal
marginal zone.
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