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Figure 7


Fig. 7. Long microfilament bundles parallel the equatorial shear. A parthenogenetically activated egg treated with BDM/nocodazole observed to be undergoing torsion (A) was fixed and stained with rhodamine-phalloidin and examined via confocal microscopy (B). Bundles of cortical microfilaments parallel the direction of shear in the equatorial region. The region indicated by the colorized box is shown at higher magnification (C). A small post-fixation tear in the surface (dark spot) reveals relatively little organized f-actin beneath the cortex. Scale bar in C: 30 µm.





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