DEV02651 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 -

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• Supplemental Figure 1 -

  Fig. S1. Abnormal morphology of the ovary isolated from 10-month-old sterile founder female. Transgenic founder female was sacrificed at 10 months of age and ovaries were fixed, paraffin embedded and stained with hematoxylin and eosin. WT (A) and TG (B) ovaries are shown. (C-E) High magnification images of the TG ovary in B, showing abnormal epithelial and fibrous tissue. (F) Vacuolated granulosa cells from the same ovary. Scale bars: in B, 100 μm for A,B; in F, 20 μm for C-F.

• Supplemental Figure 2 -

  Fig. S2. Low expression of GFP in GV stage oocytes results in incomplete destruction of CPEB. RNA isolated from 10 GV stage oocytes was reverse transcribed and PCR amplified. The level of GFP expression in the fully-grown oocytes is significantly lower than in the whole ovary. There is also significantly more of the Cpeb mRNA (compare with Fig. 2B).