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Fig. 3. Post-pachytene knockdown of Cpeb results in oocyte
abnormalities. Ovaries collected from animals at 2, 4 and 6 months of age
were processed for histology as described above. (A) An oocyte in a
follicle (stage 5-6) that was detached from the cumulus granulosa cells
(*) with abnormal and improperly positioned tripolar spindles
(arrow). (B) The oocyte had a disorganized rosette of chromosomes
(arrow) at MI that were spread randomly among three spindle poles (bold
arrow). (C) This oocyte, enclosed in the asymmetrically developed
granulosa cells (one to five layers), in a stage 5a follicle, contained a
monopolar spindle; the granulosa cells were also starting to detach.
(D-F) These pre-antral stage follicles contained MI and MII stage
oocytes that displayed apparent granulosa cell apoptosis. (D) This oocyte had
improperly extruded polar bodies at opposite sides, and displayed premature
antrum formation (*). (E) This oocyte contained a polar body with
condensed chromosomes forming a metaphase plate and a spindle. (F) The oocyte,
enclosed in a pre-antral follicle, contained a polar body with three
pronucleus-like structures. (G-I) These oocytes contained poorly
structured nuclei and/or nuclear membranes. (G) This stage 3a oocyte lacked a
nuclear membrane and contained partially condensed chromatin. Notice the
chromatin particles dispersed in the oocyte cytoplasm. (H) This fully
developed oocyte lacked normal nuclear membrane and contained several
cytoplasmic structures that resemble nuage or perhaps nucleoli (empty
arrowheads, also in I). (I) This fully developed oocyte had a disassembled
nuclear envelope with condensed chromatin in the cytoplasm. Scale bars: 10
µm in G; 20 µm in all other panels.
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