DEV02648 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Expression of C. elegans pak-1 and pak-2. (A-C) Expression pattern of pak-1 from znIs5Ppak-1::GFP transgenic animals. The promoter element contains the 3.2 kb region from 5270 to 2116 of cosmid C45B2 (A) GFP expression in a fourth larval stage (L4) animal is high in the VCCMNs (arrows) and their commissures (arrowheads), the pharynx, migrating distal tip (DTC) cells and the developing uterus. (B) In an L4 transgenic animal, GFP can be clearly seen in the commissures (arrowhead) of the ventral cord motor neurons (arrow). (C) A region of the image shown in A, focusing on the developing uterus region and the DTC (arrowhead). (D-F) Expression pattern of pak-2 from znIs6Ppak-2::GFP transgenic animals. The promoter element contains the 5.3 kb region from 7912 to 2624 of cosmid C45B11 (D) An L1 transgenic animal shows no evidence of neuronal expression. (E) GFP expression is high in the pharynx (arrow) of a young adult animal. (F) In young adults, GFP expression can also be observed in the vulva and spermatheca. In all images, anterior is to the left, dorsal is up. Scale bar: 10 μm.

• Supplemental Figure 2 -

  Fig. S2. A model for PAK activity during P cell migration and ventral cord commissural motor neuron axon guidance. (A) A model for PAK activity in the postembryonic migration of the P cell ectoblasts. The PAKs function in a GEF-rac-PAK signaling cascade. An as yet unknown receptor activates the GEF UNC-73/Trio, which then activates through both rho and rac signaling cascades. (B) A model for PAK activity during the dorsally directed guidance of the VCCMN axons. The PAKs function downstream of a GEF-rac signaling cascade during VCCMN axon guidance. This signaling module is likely to be acting downstream of multiple signaling cascades. MAX-2 has an additional, rac-independent role during VCCMN guidance downstream of the UNC-6/netrin receptor UNC-5.