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Fig. 3. Genetic and physical interactions between Smg and the CCR4-NOT complex
of deadenylation. (A) Genetic interaction between smg and
twin. Females of the indicated genotypes were crossed with wild-type
males, and hatched and unhatched embryos were scored. (B) PAT assays of
nos and control sop mRNAs showing a wild-type pattern of
nos mRNA poly(A) tails in embryos from smg1/+ or
twin8115/+ females and a stabilization of nos
mRNA with longer poly(A) tails in embryos from smg1/+
twin8115/+ double heterozygous females; poly(A) tails were
still longer in embryos from double homozygous smg1
twin12209 mutant females (right panel). Females were crossed
with identical males. (C) Co-immunoprecipitation of CAF1 protein with
Smg in 0-3 hour embryo extracts. Proteins were immunoprecipitated with
anti-Smg (Smg IP) or rabbit serum (mock IP) either in the presence or the
absence of RNase A. Bound proteins were detected by western blots with
anti-Smg or anti-CAF1. Extract before IP was also loaded.
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