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Figure 4


Fig. 4. Deadenylation of nos mRNA by CCR4 contributes to its translational repression. (A) Immunostaining of embryos with anti-Nos antibody during the first hours of embryogenesis. The increase in amounts of Nos protein was visible in bulk embryos from twinKG877/Df(3R)crb-F89-4 and twin12209/Df(3R)crb-F89-4 females. twin mutant females were crossed with identical males and smg1/Df(ScfR6) females were crossed with wild-type males. (B-D) Cuticle preparations of embryos showing strong head defects. (B) Wild type. (C,D) Embryos from twin12209/Df(3R)crb-F89-4 females crossed with wild-type males; (C) head replaced by a hole; (D) no head structures. Anterior is oriented toward the left.





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