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Fig. 10. Hand1 regulates cardiomyocyte proliferation versus differentiation in
vitro via G1 cell-cycle progression or exit. (A) Myocardial beating
in embryoid bodies (differentiated for up to 14 days) derived from wild-type
(+/+), Hand1-null (-/-), control (responder transgene alone, co) and
Hand1-overexpressing (oe) ES-cell lines. (B) Quantitative
real-time PCR analysis on RNA extracted from embryoid bodies following 14 days
of differentiation, for an array of cardiac markers. (C) Western blots
of day-14 EBs using antibodies for cyclin D2, Cdk4 and Gapdh. (D)
Quantification of protein levels from C normalised to Gapdh using scanning
densitometry.
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