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Fig. 3. Primary heart tube expansion in Hand1-overexpressing embryos at
E8.0-8.5. Bright-field whole-mount left lateral views of hearts from
somite-matched Hand1-overexpressing (oe) embryos and control (co)
littermates dissected pre-embryonic turning (nine somites; A,B)
and during turning (12 somites; C,D). Haematoxylin and Eosin
stained frontal sections through the heart tube of a control (E-H) and
overexpression embryo (I-L) at the 12-somite stage. Whole-mount in situ
hybridisation for Hand1 on control (M,O) and
overexpression embryonic hearts (N,P). (A,B) The primary heart
tube pre-turning is expanded (white arrowheads) and early stages of looping
are accentuated in overexpressing embryos compared with control. Extension and
exaggerated ventral and left lateral displacement of the heart tube can be
seen as looping progresses during turning (depicted by white arrows; C,D;
compare E-H with I-L). (M-P) Confirmation of appropriate spatial
overexpression of Hand1 in the mutant embryos at the 12-somite stage.
co, control; oe, overexpression; s, somites. Anterior (A) - posterior (P) and
left (L) - right (R) axes are indicated for E-L. Scale bar: in D,P, 50 µm
for A-P.
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