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Fig. 7. Markers of cardiomyocyte differentiation are down regulated in
Hand1-overexpressing embryos. RNA in situ hybridisation analysis
of Anf and Wnt11 in control embryos
(A,C,E,G,I) and moderate phenotype
Hand1-overexpressing embryos
(B,D,F,H,J) at E9.5. Sagittal sections of
the control (C,I) and Hand1-overexpressing hearts (D,J). Boundary of
Anf expression in proximal outflow tract of overexpressing embryos is
depicted by a broken red line in F. Wnt11 in distal outflow tract
(black and white arrowheads) is downregulated in overexpressing embryos (H,J)
compared with control embryos (G,I). at, atrium; co, control; do, distal
outflow tract; lv, left ventricle; oe, overexpression; oft, outflow tract; po,
proximal outflow tract. Scale bar: 100 µm in A.
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