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Fig. 1. Eye-field morphogenesis during forebrain neurulation.
(A-D,I-L) Projections of GFP-labelled nuclei (also see Movie 1
in the supplementary material). Dorsal projection, anterior is left.
(E-H,M-P) 3D projections of tracked cells (also see Movies 2 and
3 in the supplementary material). Frontal projection, dorsal is up. (A,E)
Forebrain regions prior to neurulation (midline, broken line). (B,F) Anterior
neural plate contracts posteriorly towards the hypothalamic tip, where neural
keel formation commences (asterisk). (C,G) Subducting hypothalamus moves
anteriorly beneath the medial eye field. Convergence narrows the neural plate,
which begins to fold lateral to medial, forming a neuropore (broken line).
Posterior eye field moves anteriorly. (D,H) Hypothalamus emerges anterior and
ventral, eye field remains contiguous across midline. (I,M) Eye evagination
begins with cells moving into vesicles as posterior-lateral optic stalk and
diencephalon converge and move anteriorwards. (J,N) Medial eye field continues
to move into eye vesicles. (K,O) Lateral telencephalon meets at the midline to
close the neuropore; anterior eye-field splitting is complete (arrows).
Posterior optic stalk arrives beneath anterior stalk precursors. (L,P) Eye
tissue now within eye vesicles. hpf, hours post fertilisation. Asterisks
indicate anterior tip of neural keel formation (hypothalamus). Arrows indicate
the direction of movement of the cells. Time (bottom left-hand corner) in hpf.
Colours are indicated in A. A, anterior; P, posterior. Scale bars: 25
µm.
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