DEV02668 Supplementary Material

Files in this Data Supplement:

• Supplemental Table - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Cell autonomy of sox10 action in DRG sensory neurons. Fluorescein dextran-labelled wild-type donor cells were transplanted into unlabelled sox10 mutant host embryo, and differentiated sensory neurons identified by anti-Hu immunofluorescence (red) at 3 dpf. Embryo is shown as a projected confocal stack. Transplanted cells (green) contributed to three adjacent DRGs, but whereas Hu+ neurons are found in two of them, the third (*) lacks sensory neurons despite having a large contribution of wild-type cells to glial component. Note that all other fluorescein+ cells lie within the CNS. Among our transplants, two separate individuals showed a result equivalent to that documented here. Thus, wild-type glia are insufficient for survival of DRG sensory neurons.

• Supplemental Figure 2 -

  Fig. S2. Proliferating neurons in wild-type and sox10 mutant DRGs. (A) Double immunofluorescence confocal image (single focal plane) of 72 hpf wild-type embryo showing two adjacent DRGs in the anterior tail. Three Hu+ neurons (green) are visible, two expressing phospho-histone H3 (pH3, red). (B) Double immunofluorescence confocal image (single focal plane) of 72 hpf sox10 mutant embryo showing DRGs in the anterior tail. One Hu+ neuron is expressing pH3. An additional DRG is also visible out of focus to the right. Scale bar: 50 μm.