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Fig. 2. Glial differentiation fails in sox10 embryos.
(A,B) Double immunofluorescent detection of trunk sensory
neurons (Hu, red) and prospective glial cells (GFP, green) in anterior trunk
in 5 dpf wild-type (A) and sox10 mutant (B) embryos carrying the
sox10:egfp transgene. Although GFP+ cells occupy similar
positions in sox10 mutant and wild-type embryos (arrows), the
elongated morphology of Schwann cells and the compact clustered arrangement of
satellite glial cells in DRGs (arrowheads) of wild-type embryos (A) are absent
in sox10 mutants (B). Neurons are often displaced ventrally in
sox10 mutant embryos, but sensory neuron number may be approximately
normal at this axial position (B). (C,D) In situ hybridisation
with mbp reveals absence of differentiated Schwann cells in
sox10 embryos (D) compared with wild-type siblings (arrowhead, C).
Likewise, oligodendrocyte differentiation (*, C) fails in
sox10 mutants (D). Scale bars: 20 µm in A; 50 µm in B.
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