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Fig. 3. sox10 mutants display a DRG neuron phenotype.
Immunofluorescent detection of Hu at 5 dpf (A,B), DM-GRASP (zn-5
antibody) at 8 dpf (D,E) and Isl-1 at 60 hpf (F,G)
in wild-type (A,D,F) and sox10 (B,E,G) embryos demonstrates the
reduction in DRG sensory neurons (arrowheads) in the mutant. Quantitation
(mean+s.d.) of Hu (C) and Isl-1 (H) phenotypes shows increasing
severity posteriorly. Significant reduction in sensory neurons is noted at all
stages (** P<0.01, ***P<0.0001;
Student's t-test). n=12 embryos for each of wild-type and
sox10 mutants in (C) and n=15 (wild type) and n=13
(sox10) in (H). There is an absence of enteric neurons in
sox10 mutants (arrow, B). However, DM-GRASP expression in secondary
motorneuron processes (*,D) is unchanged in sox10 mutants
(E). Likewise, Islet1 also labels ventral spinal cord motorneurons
(*,F,G). Quantification (I) of these cells at 5 dpf per
somite segment equivalent shows no significant difference between wild-type
and sox10 embryos (P=0.609; Student's t-test).
Three consecutive segment-lengths in the tail were counted in seven embryos of
each genotype. Scale bars: 200 µm in A,B; 50 µm in D-G.
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