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Figure 1


Fig. 1. Genetic analysis of mir-84. (A) Sequence alignment of the let-7 family members of C. elegans. Residues identical between mir-84 and let-7 are shown in bold. Boxes indicate residues conserved among all let-7 paralogs. (B) The mir-84 genomic region. Numbers correspond to cosmid B0395 (Accession number: emb|Z68131). The dashed line indicates the extent of deletion tm1304. Arrows indicate mature mir-84 and related primers. (C,D) Northern blots showing mir-84 levels in early L4-stage larvae of the indicated genotypes. A shorter exposure time was used in D than in C. Levels of 5S rRNA, stained by ethidium bromide, or U6 snRNA, provide loading controls.





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